The Lightner Museum is located in historic downtown St. Augustine, Florida. It is 20 minutes from I-95, one hour from Jacksonville and Daytona Beach, and two hours from Disney World, Epcot Center and Cape Canaveral. The museum is handicapped and wheelchair accessible. Last Admission: 3:30 p.m. The museum is closed Thanksgiving Day, Christmas Day, and New Year's Day. Hours may vary for other holidays, such as Christmas Eve.